Journal: Microorganisms

Article Title: Inactivation of the sfgtr4 Gene of Shigella flexneri Induces Biofilm Formation and Affects Bacterial Pathogenicity

doi: 10.3390/microorganisms8060841

Figure Lengend Snippet: S. flexneri forms biofilm and deletion of either SfpgdA or Sfgtr4 accelerates this formation. ( A ) Quantification of biofilm formation by crystal violet (CV) assay. Bacteria were grown in tryptic casein soy broth (TSB) medium during 24 h at 37 °C and Biofilm formation was monitored by measuring the absorbance at 570 nm. Strains used are WT, mutants SfpgdA , Sfgtr4, phoP , and BS176 (pWR100 cured S. flexneri strain). Biofilm formation was also measured using the biofilm ring test after bacterial incubation at 37 °C during 5 h ( B , D ) and 6 h ( C ). ΔBFI (Biofilm Formation Index) represents (BFI controls−BFI samples). Plasmids pKA1(SfPgdA) and pKA2 (SfGtr4) were used in the complementation experiments. Wild-type P. aeruginosa (PAO1) and BHI medium were used as positive and negative controls, respectively. Shown data are the mean of three independent experiments, and each one was performed in triplicate ( n = 3).

Article Snippet: Bacteria were grown in tryptic casein soy broth (TSB, Becton Dickinson, Belgium) at 37 °C.

Techniques: Bacteria, Incubation